mouse timp1 duoset elisa kits  (R&D Systems)

Journal: Genes & diseases

Article Title: Chemerin deficiency regulates adipogenesis is depot different through TIMP1.

doi: 10.1016/j.gendis.2020.04.003

Figure Lengend Snippet: Figure 4 TIMP1 secreted by macrophage is involved in the regulation of adipogenesis. (A) Concentration of TIMP1 in supernatant of CD45þ cells in indicated depots of mice. (B) Concentration of TIMP1 were quantified. (C) Concentration of TIMP1 in supernatant of CD45þ cells in indicated depots of mice. (D) Oil red O stain of 3T3-L1 in different concentration of TIMP1. (E) mRNA expression of TIMP1 in adipocyte and macrophage. (F) Representative IHC staining of F4/80 in SAT and VAT from WT and Rarres2/ mice. All the mice were male and fed with HFD for 12 weeks if not indicated otherwise, n Z 6e8/group. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: TIMP1 concentrations in supernatants of CD45þ immune cells were analyzed by Mouse TIMP1 DuoSet Elisa kits from R&D system according to the manufacturer’s protocol.

Techniques: Concentration Assay, Staining, Expressing, Immunohistochemistry

Journal: Genes & diseases

Article Title: Chemerin deficiency regulates adipogenesis is depot different through TIMP1.

doi: 10.1016/j.gendis.2020.04.003

Figure Lengend Snippet: Figure 5 Knock down of chemerin specific in SAT promote adipogenesis and improve GTT and ITT. Male mice were fed with HFD and infected with shLacZ or shRarres2 twice weekly via subcutaneous injection adjacent to inguinal SAT for 8 weeks. (A) Gene expression levels of chemerin in SAT. (B) Body weight of mice. (C) Comparison of SAT. (D) Ratio of fat weight to body weight. (E) Total DNA of SAT. (F and H) Adipocyte cell size of SAT. (F and G) IHC of TIMP1 in SAT. (I) mRNA expression levels of TIMP1 in SAT. (J) Western blot analysis of PPARg and C/EBPa. (K) GTT and ITT analysis. (L-M) Representative flow cytometric analysis of adipocyte- progenitors (L) and macrophage (M) in SAT of indicated mice. n Z 6/group. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: TIMP1 concentrations in supernatants of CD45þ immune cells were analyzed by Mouse TIMP1 DuoSet Elisa kits from R&D system according to the manufacturer’s protocol.

Techniques: Knockdown, Infection, Injection, Gene Expression, Comparison, Expressing, Western Blot

Journal: PLoS ONE

Article Title: Integrated Proteomic Analysis of Human Cancer Cells and Plasma from Tumor Bearing Mice for Ovarian Cancer Biomarker Discovery

doi: 10.1371/journal.pone.0007916

Figure Lengend Snippet: Protein levels of TIMP1, LCN2, PFN1, IGFBP2, SPARC, EEF1B2 and CLU were determined from the following: (A) normal ovarian tissue (N) and ovarian tumors (T) isolated from the K-ras/Pten, or (B) Pten/Apc mouse model of ovarian cancer, (C) conditioned media (CM) from normal human surface epithelium cells lines (HOSE) and ovarian cancer cell lines and (D) human primary tissues (HPT): normal ovarian tissue (N) and ovarian tumors (T). For mouse preparations, tissue lysates were prepared from normal ovaries and tumors taken from the same animal (a–d) along with four-five tumors from separate animals (e–i). For human primary tumor (HPT) samples, tissue lysates were prepared from 4 tumor samples paired with their respective normal tissue from the same patient and 3 lone tumor samples, in which no normal samples were available. Patient samples are as follows: patient #14 had independent bilateral serous borderline tumors in the left (TL) and right ovary (TR), tumors #3, #7, and #15 are papillary serous carcinomas, tumor #12 is an epithelial borderline tumor of the Müllerian-type, with endocervical mucinous and serous differentiation, tumor #13 is clear cell carcinoma, and tumor #16 is endometrioid adenocarcinoma.

Article Snippet: Timp1 concentrations in murine plasma were measured using a Quanitikine-Mouse Timp1 ELISA Kit, while mouse Lcn2 levels were detected with a DuoSet ELISA Kit (R&D Systems, Minneapolis, MN USA).

Techniques: Isolation

Journal: PLoS ONE

Article Title: Integrated Proteomic Analysis of Human Cancer Cells and Plasma from Tumor Bearing Mice for Ovarian Cancer Biomarker Discovery

doi: 10.1371/journal.pone.0007916

Figure Lengend Snippet: TIMP1 and LCN2 levels in murine plasma at various stages of tumor progression were determined by ELISA as described in the (A, B). Also shown are TIMP1 (C) and LCN2 (D) levels in murine ovarian tumor fluid and peritoneal ascites as compared to plasma. Statistical significance was determined using a two-tailed Student's t-test *p<0.05, **p<0.01, and *** p<0.0001 compared to controls.

Article Snippet: Timp1 concentrations in murine plasma were measured using a Quanitikine-Mouse Timp1 ELISA Kit, while mouse Lcn2 levels were detected with a DuoSet ELISA Kit (R&D Systems, Minneapolis, MN USA).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: PLoS ONE

Article Title: Integrated Proteomic Analysis of Human Cancer Cells and Plasma from Tumor Bearing Mice for Ovarian Cancer Biomarker Discovery

doi: 10.1371/journal.pone.0007916

Figure Lengend Snippet: Proteins increased in human ovarian cancer patient samples compared to control samples in Set 1 * (p <0.05).

Article Snippet: Timp1 concentrations in murine plasma were measured using a Quanitikine-Mouse Timp1 ELISA Kit, while mouse Lcn2 levels were detected with a DuoSet ELISA Kit (R&D Systems, Minneapolis, MN USA).

Techniques: Control, Membrane